Top Five Reasons to Automate Manual Pipetting
#1 Increased Accuracy and Precision
In terms of liquid handling, accuracy refers to how closely the measured volume reflects the intended volume, while precision describes the closeness of multiple iterations of the same volume. Despite researchers’ best efforts, there will always be some degree of human error associated with manual pipetting. By minimizing intra- and inter-operator variability, automated pipetting systems increase accuracy and precision for more dependable results.
To put this into context, increased accuracy and precision equates to greater experimental consistency, which is critical for methods such as PCR/qPCR, where consistent amplification relies on highly precise dispensing of small volumes of reagents and samples. Increased accuracy and precision also mean better experimental reproducibility, which is of paramount importance for tests that will be run numerous times.
#2 Save time
Time spent on tedious and repetitive manual pipetting tasks, such as performing multiple serial dilutions or aliquoting reagents into large numbers of vessels, can add up quickly. As a consequence, researchers are prevented from carrying out other essential tasks, like data analysis and grant-writing, which can lead to project timelines being delayed.
By acting as an extra pair of hands, automated pipetting systems offer more walk-away time in which to do more science, thus increasing the potential for novel discoveries. Today, most manual pipetting techniques can be easily programed using modern automation. And, even if projects change, writing new protocols or adapting existing protocols is usually straightforward.
#3 Improved Ergonomics and Safety
Manual pipetting can cause repetitive strain injuries and fatigue, and can mean that researchers are directly exposed to hazardous substances. For example, when performing next-generation sequencing (NGS), the workflow spanning sample prep through to library creation can involve hours of hands-on time and necessitate handling multiple chemistries.
Not only do automated pipetting systems decrease the physical burden on laboratory personnel, but they can also help limit exposure to potentially harmful chemicals and biological substances. By introducing automation into laboratory workflows, it is possible to both reduce strain and better protect the health and safety of lab workers.
#4 Enhanced Data Quality and Integrity
Contamination represents a major risk for many research applications, especially PCR/qPCR, where it can cause excessive or unexpected signal. Depending on the application being performed, contamination can come from the laboratory environment, personnel, or other samples being handled at the same time, and can lead to data being incorrectly interpreted and/or experiments needing to be repeated.
Automated pipetting systems minimize the risk of contamination due to their closed and controlled environments, which may incorporate HEPA filtration and laminar flow, as well as by providing more controlled dispensing to prevent splashing. This contributes to enhanced data quality, while also maximizing proper use of expensive chemicals and reagents.
Another way in which automated pipetting systems enhance data quality is through having integrated software that logs data. This offers better traceability and accountability for regulatory compliance and quality control, which is especially important for clinical diagnostics and regulated products, where troubleshooting and traceability is mandatory in case of any recalls or quality concerns.
#5 Scalability and Flexibility
Increasing throughput, performing diverse assays, and introducing new applications can involve significant effort when undertaken manually. In contrast, automated pipetting systems can be customized to fit a variety of laboratory workflows, accommodating different volumes, liquid types, and assay formats, allowing for seamless integration into existing processes and easy adjustments as project needs evolve.
This adaptability can be particularly beneficial for core labs that are continually incorporating new assays and for start-ups that are constantly looking to grow and increase efficiency. More generally, automation also serves to future-proof a lab, letting end users easily expand their research scope or adopt new techniques without significant additional investment.
The Liquid Handling Station (LHS) pipetting robot from BRANDTECH Scientific represents an ideal entry point into laboratory automation, enabling the movement of volumes from 1µL to 1mL. With a small footprint, intuitive software, and easy changeover between single channel and multichannel pipetting, the LHS supports a broad range of research applications. To learn more about the LHS, visit brandtech.com/LHS